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Kaixin micro test
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Test probe P100-M3
Human Interferon Beta (IFN-β/IFNB) ELISA Kit Instruction Manual
ElisaKit Specifications: Available in 48-well or 96-well configurations.
Standard Dilution: 1.5ml × 1 bottle.
Enzyme Standard Reagent: 3ml × 1 bottle (for 48-well) or 6ml × 1 bottle (for 96-well).
[Human Beta Interferon (IFN-β/IFNB) ELISA Kit] This reagent is for research use only and not for human or animal consumption.
Calculation Method:
Plot the standard concentrations against their corresponding OD values to create a standard curve. Use this curve to determine the concentration of the sample based on its OD value. Multiply by the dilution factor to obtain the actual concentration. Alternatively, calculate the linear regression equation from the standard curve and plug in the sample’s OD value to compute the final concentration.
Kit Composition:
- Sealing Film: 2 pieces (48) / 2 pieces (96)
- Instructions: 1 copy
- Sealed Bag: 1
- Standard: 2700ng/L, 0.5ml × 1 bottle, stored at 2-8°C
- Enzyme Label Coated Plate: 1×48 or 1×96, stored at 2-8°C
- Sample Dilution: 3ml × 1 bottle (48) or 6ml × 1 bottle (96), stored at 2-8°C
- Reagent A: 3ml × 1 bottle (48) or 6ml × 1 bottle (96), stored at 2-8°C
- Developer B Solution: 3ml × 1 bottle (48) or 6ml × 1 bottle (96), stored at 2-8°C
- Stop Solution: 3ml × 1 bottle (48) or 6ml × 1 bottle (96), stored at 2-8°C
- Concentrated Washing Solution: (20ml × 20 times) × 1 bottle (48) or (20ml × 30 times) × 1 bottle (96), stored at 2-8°C
Experimental Principle:
This kit utilizes the sandwich ELISA method to quantify Human Interferon Beta (IFN-β/IFNB) in samples. The microwell plate is pre-coated with a specific antibody against IFN-β. After incubation with the sample and HRP-labeled secondary antibody, an immune complex is formed. TMB substrate is added, producing a color change that is proportional to the IFN-β concentration. The reaction is stopped, and absorbance is measured at 450 nm to calculate the sample concentration using a standard curve.
Purpose:
The kit is designed to measure the levels of IFN-β in human serum, plasma, urine, cell culture supernatants, tissue homogenates, and other biological fluids.
Guarantee:
Delivery period: From payment to shipment.
Free technical support during working hours. Contact us for assistance.
Sample Testing Service:
We offer free sample testing to help ensure accurate experimental results.
Storage Conditions and Expiry:
- Storage: 2–8°C
- Shelf Life: 6 months
Sample Preparation and Handling:
1. Serum: Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant; if precipitate forms, re-centrifuge.
2. Plasma: Use EDTA or sodium citrate as anticoagulant. Mix for 10–20 minutes, then centrifuge for 20 minutes at 2000–3000 rpm. Collect the supernatant.
3. Urine: Collect in a sterile tube and centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant.
4. Cell Culture Supernatant: Centrifuge at 2000–3000 rpm for 20 minutes after collection. For intracellular components, lyse cells via freezing/thawing and centrifuge again.
5. Tissue Specimen: Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge at 2000–3000 rpm for 20 minutes. Store remaining portions at 2–8°C.
6. Handle samples promptly after collection. If not tested immediately, store at –20°C but avoid repeated freeze-thaw cycles.
7. Avoid using samples containing NaN3, as it may inhibit HRP activity.
Procedure:
1. Prepare standard dilutions and load them into the ELISA plate.
2. Load the samples with appropriate dilution.
3. Incubate the plate at 37°C for 30 minutes.
4. Wash the plate with diluted washing solution (30x for 48-well, 20x for 96-well).
5. Add enzyme-labeled reagent to all wells except blank controls.
6. Incubate again for 30 minutes.
7. Wash the plate again.
8. Add developer solution and incubate at 37°C for 15 minutes.
9. Add stop solution to terminate the reaction.
10. Measure OD at 450 nm within 15 minutes of adding the stop solution.
Notes:
1. Let the kit equilibrate at room temperature for 15–30 minutes before use. Store unopened plates in sealed bags.
2. Crystallized washing solution can be dissolved by heating in a water bath without affecting results.
3. Use a pipette for accuracy. Keep loading time under 5 minutes.
4. Always include a standard curve and perform duplicate measurements. If sample OD exceeds the first standard well, dilute the sample before testing.
5. Use a new sealing film for each experiment to prevent contamination.
6. Protect the substrate from light.
7. Follow the manual strictly. Rely on microplate reader readings for accurate results.
8. Treat all waste materials as biohazardous.
9. Do not mix reagents from different batches.
10. In case of discrepancy, the English manual takes precedence.
Kit Performance:
- Correlation coefficient (R) ≥ 0.95
- Intra-batch variation < 9%, Inter-batch variation < 11%
Testing Range:
0.2 IU/L – 6 IU/L
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