**Tech Detail Description:**
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Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22
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**In Vitro Cell Culture Tips and Prevention Measures:**
Cell culture is a delicate process that requires strict control to prevent contamination. Once contamination occurs, it can be very difficult to recover, even with all possible efforts. Therefore, prevention is the most important step in maintaining a clean and healthy cell culture environment.
Here are some effective strategies to prevent contamination during cell culture:
1. **Sterilization of Equipment and Supplies:**
All materials used in cell culture must be thoroughly cleaned and sterilized. Solutions should be properly filtered and tested before use. The COâ‚‚ incubator should be regularly disinfected. One common method is to wipe the surfaces with 75% alcohol and then expose them to UV light for at least 30 minutes. Additionally, you can add saturated copper sulfate solution to the water tank to maintain humidity and enhance disinfection.
2. **Use of Antibiotics (with Caution):**
While antibiotics can help prevent microbial growth, overuse may lead to resistance. It’s better to use them as a preventive measure rather than after contamination occurs. If used, they should be changed periodically to avoid drug-resistant strains.
3. **Operator Hygiene and Technique:**
Operators should wash hands thoroughly before entering the sterile room and wear appropriate protective gear. Before starting work, use 75% alcohol to wipe hands, bottle mouths, and other surfaces. Avoid talking or coughing during procedures, and always work near a flame when handling open containers. Never burn metal tools for too long, and never re-use pipettes that have come into contact with culture media.
4. **Preventing Cross-Contamination:**
All cell lines should be stored in frozen stocks. If cross-contamination is suspected, genetic testing can help identify the issue. Important cell lines should be handled by two people to ensure accuracy and safety.
5. **Disinfection of Sterile Rooms:**
Regular cleaning using disinfectants like chlorpheniramine is recommended. For deep disinfection, formaldehyde fumigation can be used. Mix 10–15 ml of formaldehyde per cubic meter of space and let it sit for at least 4 hours. Afterward, neutralize the odor with ammonia water. Always wear a gas mask during this process due to the toxicity of formaldehyde.
Maintaining a clean and controlled environment is essential for successful cell culture. By following these precautions, researchers can significantly reduce the risk of contamination and improve the reliability of their experiments.
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