How to prevent pollution problems in cell culture - Database & Sql Blog Articles
In vitro culture When cell cultures become seriously contaminated, it can be extremely difficult to recover them even with all available methods. This makes prevention the most critical step in avoiding contamination. Effective preventive measures should be applied throughout the entire cell culture process to reduce the risk of microbial infiltration. Here are some general strategies for preventing contamination: 1. Sterilization of equipment and materials: All items used in cell culture must be thoroughly cleaned and sterilized before use. Solutions should be carefully filtered and tested after filtration to ensure they remain uncontaminated. Regular disinfection of CO₂ incubators is also essential. For example, after wiping with 75% alcohol, a UV lamp should be used for at least 30 minutes. To maintain humidity inside the incubator, you can add distilled water or a copper sulfate solution (prepared by dissolving 300 ml of saturated copper sulfate into 3 liters of ultrapure water). 2. Use of antibiotics: While antibiotics can help prevent contamination, their overuse may lead to resistant microorganisms. It's better to use a combination of antibiotics rather than one type, and to apply them preventatively rather than after contamination has occurred. However, it’s important to rotate antibiotics regularly to avoid resistance and minimize effects on the cells themselves. 3. Operator hygiene and behavior: Operators should wash hands thoroughly before entering the sterile room and wear appropriate protective clothing. Before starting any procedure, use 75% alcohol to wipe hands and the bottle neck. Avoid talking or coughing during operations to prevent airborne contamination. Also, avoid opening bottles too early, and keep the culture medium tilted to prevent bacterial settling. After finishing, clean the work surface with disinfectant-soaked gauze. 4. Preventing cross-contamination between cell lines: All cell lines should be stored in frozen form from the beginning. If cross-contamination is suspected, cytogenetic testing can identify changes in the genetic material. Important cell lines should be handled by two people independently to reduce the risk of error or contamination. 5. Disinfection of the sterile room: A thorough cleaning of the sterile room is crucial. New Clean, which is more cost-effective than alcohol, can be used for this purpose. Alternatively, formaldehyde fumigation is an effective method. For fumigation, use 10–15 ml of formaldehyde per cubic meter of space. Mix with potassium permanganate and allow the fumes to dissipate. Afterward, neutralize the odor with ammonia water. Always wear a gas mask when handling formaldehyde due to its toxicity. By following these practices, you can significantly reduce the risk of contamination and ensure the integrity of your cell cultures. For Pixel Glass,Pixel Glass,Google Pixel 4 Xl Glass,Google Pixel 6 Pro Front Glass Dongguan Jili Electronic Technology Co., Ltd. , https://www.jlglassoca.com