The experimental results of ELISA kits can be influenced by a variety of factors. To ensure accurate and reliable outcomes, it's essential to maintain strict quality control throughout each step of the process. Our company's experienced technicians have carefully analyzed the performance of our ELISA kits to help you better understand their advantages. Below, we share the results of our experiments.
Many customers prefer importing ELISA kits from abroad due to brand reputation, but domestic options are also highly competitive. High-quality data, fast processing times, and user-friendly procedures are key factors in kit selection. In addition, imported kits often offer cost savings on logistics and customs clearance, with more flexible packaging options like 48T or 96T formats. For example, a 48T kit can test up to 37 samples plus 11 standard points, while a 96T kit can handle 85 samples plus 11 standards.
Shanghai Huding offers both imported and domestic ELISA kits at competitive prices. We also provide free agent testing services, including shipping and handling, ensuring safe and reliable delivery. If you're interested in ordering, feel free to contact us for more information.
So, how do you interpret the results after completing an ELISA experiment? Here are some practical methods:
1. **Visual Inspection**: After adding the substrate and stopping the reaction, the color in the positive wells should be significantly darker than the negative control. In competitive assays, the opposite is true — the negative control should appear darker. A close match between the negative control and the sample indicates a negative result.
2. **OD Value Threshold**: If the OD value of the sample well is greater than the negative control's OD value plus 2–3 standard deviations (SD), it is considered a positive result.
3. **P/N Ratio**: The ratio of the positive well OD to the negative well OD should be ≥ 2.1 for a positive result. If the P/N ratio is between 1.5 and 2.1, it may indicate a suspicious result. A ratio below 1.5 is typically considered negative.
4. **Quantitative Analysis**: For quantitative ELISA results, the concentration of the target analyte in the sample is determined using a standard curve.
These are the evaluation methods used by our technical team to analyze the performance of our imported ELISA kits. If you need assistance with your ELISA experiments or want to place an order, don't hesitate to reach out. We're here to provide full guidance and support.
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