Do you know the value of the ELISA kit? -Huaqiang Electronic Network
Niimoo Innovative (HK) CO., LIMITED , https://www.niimootech.com
The experimental results of ELISA kits can be influenced by a variety of factors. To ensure reliable and accurate outcomes, it is essential to maintain high-quality control throughout each step of the process. Our company's experienced technicians have analyzed the performance of our ELISA kits and are sharing their findings with you. Below are the results.
Many customers tend to prefer imported ELISA kits due to brand reputation, but domestic alternatives are also a strong option, especially when considering cost-effectiveness and flexibility. High-quality data, combined with ease of use and shorter experimental times, makes a kit more popular among researchers. This could be an important factor when choosing an ELISA kit.
Imported kits often offer significant savings in logistics and customs costs compared to foreign reagents. They also provide more flexible packaging options, such as small quantities like 48T or 96T. For example:
- A 48T kit can test up to 37 samples plus 11 standard points.
- A 96T kit can test up to 85 samples plus 11 standard points.
Shanghai Huding offers both imported and domestically produced ELISA kits at competitive prices. We also provide free agent testing services, including shipping and handling, ensuring a safe and reliable experience for our clients.
So, how do you interpret the results after completing an ELISA experiment? Here are some key methods:
1. **Color Observation**: After adding the substrate and stopping the reaction, the positive wells should show a significantly darker color compared to the negative control (in competition assays, the opposite applies). If the negative control has a similar color, it’s likely a negative result.
2. **OD Value Threshold**: The OD value of the sample well should be greater than the negative control OD value plus 2–3 SD to be considered positive.
3. **P/N Ratio**: A P/N ratio (positive OD divided by negative OD) of ≥2.1 indicates a positive result. A ratio between 1.5 and 2.1 may suggest a questionable result, while a ratio below 1.5 is considered negative.
4. **Quantitative Analysis**: For quantitative results, the concentration of the analyte in the sample is calculated using the standard curve.
These are the key findings from our technical team’s analysis of the imported ELISA kits. If you're looking for high-quality ELISA products, feel free to contact us. We offer full guidance on ELISA techniques and are here to help you every step of the way.
